Comparison of adenosine triphosphatase activity of nuclei and mitochondria from mouse mammary adenocarcinoma.

Nuclei from a mammary adenocarcinoma and liver indigenous to the same mouse, isolated by two different methods, were shown to contain adenosine triphosphatase (ATPase) activity. The distribution of ATPase activity in subcellular portions of the transplanted mammary tumor was ascertained and found to be highest in the mitochondrial and nuclear moieties. Comparison was made between the ATPase activity of nuclei and mitochondria isolated from the same tissue with respect to the effects of pH, 2,4-dinitrophenol, ethylenediaminetetraacetate (EDTA), Mg++and Ca"1"1" cations,